Use of human intestinal organoids for theratyping in cystic fibrosis

Task 1: To measure CFTR function in organoids of individual patients undergoing treatments with 1) Ivacaftor (Kalydeco), 2) Orkambi by FIS assay. Task 2: To study CFTR expression and localization and CFTR channel in organoids. CFTR expression and subcellular localization will be studied by confocal microscopy analysis. Task 3: Evaluate all clinically relevant data recorded in the clinical database in relation to the outcome of FIS assay. Understanding the value of translational research for future clinical applications by performing cell culture activity and analysis of gene and protein expression by PCR, western blotting and immunofluorescence. According to the basic knowledge and the capability demonstrated the student he/she will be allowed to personally perform some of these tasks under the supervison of experienced personnel so he can better understand how to work in a laboratory.