Western Blotting: Best Practices, Tips and Tricks

Duringthisseminar youwillhavethe opportunityto discoverthe key tipsand tricks, and improveyourwestern blottingexperiments. Therewillbe the opportunityto optimizeyourprotocolsand askyourburningquestions.

Agenda

• Electrophoresis Doctor: most common electrophoresis problems
• Blotting Doctor: Does the perfect blot exist?
• Normalization: Why we normalize? Is it better to use HK proteins or total protein?
• Imaging: which is the best acquisition for your membrane?
• Image Analysis with ImageLab Desktop