Bio-functionalized gold nanoparticles: from design to application

Dr. Roberto Fiammengo | Team Leader - Researcher - Center for Biomolecular Nanotechnologies - Fondazione Istituto Italiano di Tecnologia (IIT) Arnesano (Lecce), Italy

13-set-2019 - Ora: 14:00 | Sala Verde - Piramide Cà Vignal - Strada Le Grazie 15, Verona

Gold nanoparticles (AuNPs) have been proposed for sensing applications,[1] in nanomedicine,[2] and to understand the interaction between engineered nanomaterials and biological systems.[3] Yet, limited efforts have been devoted to the development of biofunctionalized AuNPs with a precisely defined architecture at the molecular level. Our aim is to push forward the design, preparation, and characterization of AuNPs functionalized with peptides, proteins or oligonucleotides to enable advanced biologically-oriented applications of these materials.
In the past years, we have developed a reliable coating strategy for the surface of AuNPs, which allows excellent control of nanoparticle functionalization and of colloidal stability.[4] In fact, the desired biomolecules are immobilized at low surface density on the negatively charged, hydrophilic nanoparticle coating maximizing their bioavailability. Using this approach, we have been able to prepare AuNPs targeting specific receptors on the cell membrane,[4,5] novel anticancer vaccine candidates,[6,7] and sensitive microRNA sensing systems.[8]
In this talk, I will first present our AuNP coating approach and then describe the bioconjugation strategies we follow for the immobilization of peptides, proteins, and DNA-probe sequences. Next, I will illustrate how the molecular design of these AuNPs is indeed crucial for their performances. In particular, I will present our recent results employing AuNP-based formulations to develop mucin 1-directed anticancer vaccines, and the use of biofunctionalized AuNPs to target cell receptors on the plasma membrane.
References: [1] K. Saha, S. S. Agasti, C. Kim, X. Li, V. M. Rotello, Chem. Rev. 2012, 112, 2739-2779. [2] D. Giljohann, D. Seferos, W. Daniel, M. Massich, P. Patel, C. Mirkin, Angew. Chem., Int. Ed. 2010, 49, 3280-3294. [3] X. Yang, M. Yang, B. Pang, M. Vara, Y. Xia, Chem. Rev. 2015, 115, 10410-10488. [4] L. Maus, O. Dick, H. Bading, J. P. Spatz, R. Fiammengo, ACS Nano 2010, 4, 6617-6628. [5] V. Maggi, F. Bianchini, E. Portioli, S. Peppicelli, M. Lulli, D. Bani, R. D. Sole, F. Zanardi, A. Sartori, R. Fiammengo, Chem. Eur. J. 2018, 24, 12093-12100. [6] H. Cai, F. Degliangeli, B. Palitzsch, B. Gerlitzki, H. Kunz, E. Schmitt, R. Fiammengo, U. Westerlind, Biorg. Med. Chem. 2016, 24, 1132-1135. [7] I. Compañón, A. Guerreiro, V. Mangini, J. Castro-López, M. Escudero-Casao, A. Avenoza, J. H. Busto, S. Castillón, J. Jiménez-Barbero, J. L. Asensio, G. Jiménez-Osés, O. Boutureira, J. M. Peregrina, R. Hurtado-Guerrero, R. Fiammengo, G. J. L. Bernardes, F. Corzana, J. Am. Chem. Soc. 2019, 141, 4063-4072. [8] F. Degliangeli, P. Kshirsagar, V. Brunetti, P. P. Pompa, R. Fiammengo, J. Am. Chem. Soc. 2014, 136, 2264-2267.
Seminario organizzato nell'ambito del ciclo di seminari per la selezione dei candidati al reclutamento per il Dipartimento di eccellenza
Data pubblicazione

Paola Dominici